Journal:

Article Title: Measles Virus (MV) Nucleoprotein Binds to a Novel Cell Surface Receptor Distinct from Fc?RII via Its C-Terminal Domain: Role in MV-Induced Immunosuppression

doi: 10.1128/JVI.77.21.11332-11346.2003

Figure Lengend Snippet: MV-N binding on different cell types

Article Snippet: The cell lines 3D1 (murine cortical TEC), HeLa (epithelial human carcinoma from cervix), MeWo (fibroblastic human melanoma from lymph node), NIH 3T3 (mouse fibroblasts from embryo), Jurkat (human acute T-cell leukemia), and Vero (monkey fibroblast from kidney) were obtained from the American Type Culture Collection (Manassas, Va.).

Techniques: Binding Assay, Inhibition, Expressing

Journal: Clinical and Developmental Immunology

Article Title: Modulatory Effect of 1,25-Dihydroxyvitamin D 3 on IL1 β -Induced RANKL, OPG, TNF α , and IL-6 Expression in Human Rheumatoid Synoviocyte MH7A

doi: 10.1155/2013/160123

Figure Lengend Snippet: Effect of 1,25(OH) 2 D 3 on IL β -induced TNF α and IL-6 production in MH7A. MH7A cells were stimulated with 20 ng/mL IL1 β and then treated with different concentrations of 1,25(OH) 2 D 3 (0.1 nM, 1 nM, 10 nM, and 100 nM) for 48 h. The effects of 1,25(OH) 2 D 3 on IL β -induced TNF α (a) and IL-6 (b) mRNA expression in MH7A was analyzed by real-time PCR, and the production of IL-6 in supernatants was shown in (c). The data shown are the mean ± SD for three independent experiments, each in triplicate. * P < 0.05 compared to cells cultured with IL-1 β alone. ** P < 0.01 compared to cells cultured with IL-1 β alone.

Article Snippet: Cytokine production was determined in cell culture supernatants using ELISA specific for human IL-6 and TNF α (BOSTER-BIO; Wuhan; China) following manufacturer's guidelines.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Cell Culture